Rat IL-1 RI ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80382

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum (100 ul)

Sensitivity

14.76 pg/ml

Assay Range

54.69-3500 pg/ml (example only; lot dependent)

View all IL-1 RI ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Conjugate

HRP

Reactivity

Rat

Specificity

Recognizes both recombinant and natural Rat IL-1 RI.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 3.1

Inter-Assay Precision (Precision between assays) CV% < 8.3 %

Recovery for Rat IL-1 RI ELISA Kit (Colorimetric)

Recovery

124-129%

Scientific Data Images for Rat IL-1 RI ELISA Kit (Colorimetric)

Sandwich ELISA: Rat IL-1 RI ELISA Kit (Colorimetric) [NBP2-80382] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Kit Contents for Rat IL-1 RI ELISA Kit (Colorimetric)

  • 1. 96 well microplate coated with Capture Antibody
  • 2. Detection Antibody conjugated to HRP
  • 3. Standards
  • 4. Wash Buffer Concentrate
  • 5. Dilution Buffer Concentrate
  • 6. Color Reagent A
  • 7. Color Reagent B
  • 8. Stop Solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage is content dependent.

Background: IL-1 RI

The Interleukin 1 receptor type I (IL-1R1) belongs to the IL-1 receptor family (1). IL-1R1 binds to IL-1 alpha, IL-1 beta, and interleukin-1 receptor antagonist protein (IL-1RA). IL-1RA regulates IL-1 ligand biological activity by a competitive interaction for IL-1R1. Upon IL-1 stimulation, adaptor molecule MyD88 is first recruited to the IL-1R1-IL1R accessory protein complex, followed by the recruitment of two serine-threonine kinases, IRAK4 and IRAK, and the adaptor protein TRAF6, resulting in activation of the NF-kB pathway (2-3). Stimulation of IL-1R1, leads to activation of the transcription factors NF-B, ATF, and AP-1.

Long Name

Interleukin 1 Receptor I

Alternate Names

CD121a, IL-1RI, IL1R1, IL1RI

Gene Symbol

IL1R1

Additional IL-1 RI Products

Product Documents for Rat IL-1 RI ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat IL-1 RI ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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