Mouse IL-13 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92663

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate), buffered solution (50 ul)

Sensitivity

2.8 pg/mL

Assay Range

7.8 - 500 pg/mL

View all IL-13 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into serum. There was no crossreactivity detected, notably not with the inactive IL-13/IL-13BP complex.

Description

The mouse IL-13 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-13

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.60%

Inter-Assay Precision (Precision between assays) CV% < 5.00%

Recovery for Mouse IL-13 ELISA Kit (Colorimetric)

Recovery

The overall mean recovery of about 10% might be due to IL-13/IL13BP complex formation. Please see protocol for more specific Spike Recovery details.

Scientific Data Images for Mouse IL-13 ELISA Kit (Colorimetric)

ELISA: Mouse IL-13 ELISA Kit (Colorimetric) [NBP1-92663]

ELISA: Mouse IL-13 ELISA Kit (Colorimetric) [NBP1-92663]

ELISA: IL-13 ELISA Kit [Biotin] [NBP1-92663] - Standard curve

Kit Contents for Mouse IL-13 ELISA Kit (Colorimetric)

  • 1 bottle (10 mL) Assay Buffer A Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • 1 bottle (25 mL) Sample Diluent Z
  • 1 bottle (25 mL) Tetramethylbenzidine (TMB) Substrate Solution
  • 1 vial (100 uL) Capture Antibody Concentrate (250 x)
  • 1 vial (2.5 mL) Phosphate Buffered Saline Concentrate (PBS) 10x
  • 1 vial (50 uL) Detection Antibody Concentrate (250 x)
  • 2 96-well plates
  • 2 vials mouse IL-19 standard, (lyophilized), 8000 pg/mL upon reconstitution

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-13

Interleukin-13 is a Th2-type cytokine, secreted from CD4(+) T cells, mast cells, basophils and eosinophils. The IL-13 gene generates a cluster with other Th2-type cytokines such as IL-4 and IL-5. IL-13 is an immunoregulatory cytokine and a key mediator in the pathogenesis of allergic inflammation. It mediates its effects by interacting with a complex receptor system comprised of IL-4Ralpha and two IL-13 binding proteins, IL-13Ralpha1 and IL-13Ralpha2. IL-13 receptors are expressed on human B cells, basophils, eosinophils, mast cells, endothelial cells, fibroblasts, monocytes, macrophages, respiratory epithelial cells, and smooth muscle cells. However, functional IL-13 receptors have not been demonstrated on human or mouse T cells. Thus unlike IL-4, IL-13 does not appear to be important in the initial differentiation of CD4 T cells into T(H)2-type cells but rather appears to be important in the effector phase of allergic inflammation. The important role of IL-13 in the pathogenesis of bronchial asthma as well as other allergic diseases has been recognized, based mainly on analyses of mouse models.

Long Name

Interleukin 13

Alternate Names

IL13, NC30, P600

Gene Symbol

IL13

Additional IL-13 Products

Product Documents for Mouse IL-13 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse IL-13 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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