Mouse IL-15/IL-15R alpha Complex ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92667

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA), buffered solution (50 ul)

Sensitivity

2.0 pg/mL

Assay Range

6.3 - 400 pg/mL

View all IL-15/IL-15R alpha Complex ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

Recombinant mouse IL-15 has a cross reactivity of 2% in this assay when spiked in excess (100 ng/ml). The specificity of this ELISA has been confirmed with bone-marrow derived dendritic cells from IL-15 knock-out mice; Tissue culture supernatant from these cells following lipopolysaccharide (LPS) stimulation was negative for the presence of IL-15/IL-15R complex while wild type animals reveal more than 40 pg/ml.

Description

The mouse IL-15/IL-15R ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-15/IL-15R.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.3%

Inter-Assay Precision (Precision between assays) CV% < 6.3%

Recovery for Mouse IL-15/IL-15R alpha Complex ELISA Kit (Colorimetric)

Recovery

Please see protocol for more specific Spike Recovery details.

Scientific Data Images for Mouse IL-15/IL-15R alpha Complex ELISA Kit (Colorimetric)

ELISA: IL-15 ELISA Kit [NBP1-92667]

Kit Contents for Mouse IL-15/IL-15R alpha Complex ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-15/IL-15R
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-mouse IL-15/IL-15R polyclonal antibody
  • Calibrator Diluent
  • Mouse IL-15/IL-15R Standard lyophilized, 400 pg/ml upon reconstitution
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-15/IL-15R alpha Complex

IL-15 is a 14 kDa proinflammatory protein that has been shown to play a role in the activation of neutrophils, dendritic cells, and macrophages, and is essential to the development and survival of NK cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling through a heterotrimeric receptor. Two of the subunits of this receptor, beta and gamma, are shared with the IL-2 receptor, while the alpha subunit is unique to IL-15. Despite the expression of IL-15 mRNA in many cell types, the secreted protein is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and is only secreted in complex with its unique receptor, IL-15Ralpha. This chaperoning is required from the ER to Golgi through to secretion. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans- presented to cells expressing the beta-gamma subunits of the receptor, or may be secreted in a soluble form that lacks the transmembrane domain of the receptor. The soluble IL-15/IL-15R complex is produced by mouse dendritic cells, fibroblasts, and macrophages, and demonstrates a 10- 100-fold increase in agonistic activity over IL-15 alone.

Long Name

Interleukin 15/Interleukin 15 Receptor alpha Complex

Alternate Names

IL-15/IL-15 R alpha Complex

Gene Symbol

IL15

Additional IL-15/IL-15R alpha Complex Products

Product Documents for Mouse IL-15/IL-15R alpha Complex ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mouse IL-15/IL-15R alpha Complex ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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