Mouse IL-17/IL-17A ELISA Kit (Colorimetric)

Name: Mouse IL-17/IL-17A ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP1-92657

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92657

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Product Specifications
Precision
Recovery
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum (50 uL)

Sensitivity

1.6 pg/mL

Assay Range

7.8 - 500 pg/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a mouse IL-17 positive serum. There was no crossreactivity detected

Description

The mouse IL-17 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-17.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 9.10%

Inter-Assay Precision (Precision between assays) CV% < 8.50%

Recovery for Mouse IL-17/IL-17A ELISA Kit (Colorimetric)

Recovery

The overall mean recovery was 80%. Please see protocol for more specific Spike Recovery details.

Scientific Data Images for Mouse IL-17/IL-17A ELISA Kit (Colorimetric)

ELISA: Mouse IL-17/IL-17A ELISA Kit (Colorimetric) [NBP1-92657]

ELISA: IL-17/IL-17A ELISA Kit [Biotin] [NBP1-92657] - Standard curve

Kit Contents for Mouse IL-17/IL-17A ELISA Kit (Colorimetric)

Adhesive Films
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-17
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Biotin-Conjugate anti-mouse IL-17 monoclonal antibody
Mouse IL-17 Standard lyophilized, 1 ng/ml upon reconstitution
Sample Diluent
Stop Solution (1M Phosphoric acid)
Streptavidin-HRP
Substrate Solution (tetramethyl-benzidine)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-17/IL-17A

Mouse IL-17 mRNA is specifically expressed by activated alpha beta TCR + CD2-CD8-T-cells, a small subset with a potentially important role in immune regulation. IL-17 induces the secretion of IL-6, IL-8, PGE2, MCP-1 and G-CSF by adherent cells like fibroblasts, keratinocytes, epithelial and endothelial cells. IL-17 is also able to induce ICAM-1 surface expression of T-cells. IL-17 has been shown to support the growth of hemopoietic progenitors, it has stimulatory effects on granulopoiesis in mice. IL-17 may affect osteoclastic resorption and thereby mediate bone destruction accompanying some inflammatory diseases. Astrocytes produce NO when stimulated by IL-17, which suggests a possible role for IL-17 in the inflammatory diseases of the CNS. Forced expression of murine IL-17 induces immune responses and multiorgan inflammation. Interleukin-17 is involved in the regulation of hematopoiesis and its effects are dependent on the physiological/pathological status of the organism. IL-17 expression in airways is upregulated upon allergen exposure like in allergic asthma. Its crucial role in T-cell activation suggests that IL-17 is involved in the development of autoimmune arthritis.

Long Name

Interleukin 17

Alternate Names

CTLA-8, CTLA8, IL-17A, IL17, IL17A

Gene Symbol

IL17A

Additional IL-17/IL-17A Products

Product Documents for Mouse IL-17/IL-17A ELISA Kit (Colorimetric)

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download Manual - Mouse IL-17/IL-17A ELISA Kit (Colorimetric)

COA

SDS

Download SDS

Product Specific Notices for Mouse IL-17/IL-17A ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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