Mouse IL-17F ELISA Kit (Colorimetric)

Name: Mouse IL-17F ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP1-92666

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92666

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Product Specifications
Precision
Recovery
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum and plasma (EDTA), buffered solution (50 uL)

Sensitivity

10 pg/mL

Assay Range

62.5 - 4,000 pg/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

The assay detects both natural and recombinant mouse IL-17F. The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a mouse IL-17F positive sample. No crossreactivity to the IL-17AA homodimer is observed and less than 2.5% crossreactivity to IL-17AF heterodimer when spiked in excess (100ng/ml).

Description

The mouse IL-17F ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-17F.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.20%

Inter-Assay Precision (Precision between assays) CV% < 4.90%

Recovery for Mouse IL-17F ELISA Kit (Colorimetric)

Recovery

Please see protocol for more specific Spike Recovery details.

Scientific Data Images for Mouse IL-17F ELISA Kit (Colorimetric)

Mouse IL-17F ELISA Kit (Colorimetric) [NBP1-92666] - Mouse IL-17F ELISA Kit [NBP1-92666] - Data for Mouse IL-17F ELISA

Kit Contents for Mouse IL-17F ELISA Kit (Colorimetric)

Adhesive Films
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-17F
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Biotin-Conjugate anti-mouse IL-17F polyclonal antibody
Calibrator Diluent
Conjugate Diluent
Mouse IL-17F Standard lyophilized, 4 ng/ml upon reconstitution
Sample Diluent
Stop Solution (1M Phosphoric acid)
Streptavidin-HRP
Substrate Solution (tetramethyl-benzidine)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-17F

IL-17F and 17A are closely related members of the IL-17 cytokine family, sharing about 50% amino acid identity. Expression of IL-17F and IL-17A has been detected in activated human peripheral blood lymphocytes. It has been shown that the cytokines are expressed in activated human CD4+ T cells. Expression of IL-17F and IL-17A has also been observed in tissue samples from various autoimmune diseases such as rheumatoid arthritis, multiple sclerosis, psoriasis, inflammatory bowel disease, and asthma. The crystal structure of IL-17F has been solved and shows that the protein forms a disulfide-linked dimeric glycoprotein. IL-17A is also a disulfide-linked homodimeric glycoprotein. The IL-17F homodimer includes a classical cysteine knot motif, which is found in the TGF-beta, bone morphogenetic protein, and nerve growth factor superfamilies. One difference in the cysteine knot motif of IL-17F compared with the other known cysteine knot protein families is that it only utilizes four cysteines instead of the classical six cysteines to form the knot. IL-17F and IL-17A have been shown to form biologically active IL-17F/IL-17A heterodimers, in addition to the IL-17F and IL-17A homodimers.

Long Name

Interleukin 17F

Alternate Names

IL17F

Gene Symbol