Mouse IL-20 ELISA Kit (Colorimetric)

Name: Mouse IL-20 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-06778

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06778

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 uL)

Sensitivity

18.75 pg/mL

Assay Range

31.25 - 2000 pg/mL

View all IL-20 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This kit recognizes Mouse IL-20 in samples. No significant cross-reactivity or interference between Mouse IL-20 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 4.87

Inter-Assay Precision (Precision between assays) %CV < 5.46

Recovery for Mouse IL-20 ELISA Kit (Colorimetric)

Recovery

87 - 106%

Linearity

Scientific Data Images for Mouse IL-20 ELISA Kit (Colorimetric)

ELISA: Mouse IL-20 ELISA Kit (Colorimetric) [NBP3-06778] -

ELISA: Mouse IL-20 ELISA Kit (Colorimetric) [NBP3-06778] - Standard Curve Reference

Kit Contents for Mouse IL-20 ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-20

The class II cytokine receptor family includes 12 members, 11 of which combine as various heterodimers to transduce signals across the cellular membrane for 27 cytokines. These cytokines are divided into four structurally related groups: six cytokines of the IL-10 family, 17 type I IFNs, one type II IFN and three IFN-lamda. IL-20 is a member of the IL-10 family of cytokines that has been shown to be upregulated in psoriatic skin. The receptor for IL-20 (IL-20R) exists as a dimer consisting of IL-20Ralpha and IL-20Rbeta. IL-20R also functions as a receptor for IL-19, IL-24 and IL-26. It is highly expressed in skin, brain and testis. IL-20, IL-20Ralpha and IL-20Rbeta all have higher expression levels in psoriatic lesional (LS) skin than in nonlesional (NL) skin, suggesting a role for these proteins as potential therapeutic targets. IL-20 can induce signal transducer and activator of transcription (Stat) activation through IL-20R.

Long Name

Interleukin 20

Alternate Names

IL20

Gene Symbol