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Human IL-28B/IFN-lambda 3 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06788

Novus Biologicals, part of Bio-Techne
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NBP3-06788

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

9.38 pg/mL

Assay Range

15.63 - 1000 pg/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human IL-28B/IFN-lambda 3 in samples. No significant cross-reactivity or interference between Human IL-28B/IFN-lambda 3 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 5.03

Inter-Assay Precision (Precision between assays) 5.25

Recovery for Human IL-28B/IFN-lambda 3 ELISA Kit (Colorimetric)

Recovery

86 - 105 %

Linearity

Human IL-28B/IFN-lambda 3 ELISA Kit (Colorimetric)

Scientific Data Images for Human IL-28B/IFN-lambda 3 ELISA Kit (Colorimetric)

Human IL-28B/IFN-lambda 3 ELISA Kit (Colorimetric)

ELISA: Human IL-28B/IFN-lambda 3 ELISA Kit (Colorimetric) [NBP3-06788] -

ELISA: Human IL-28B/IFN-lambda 3 ELISA Kit (Colorimetric) [NBP3-06788] - Standard Curve Reference

Kit Contents for Human IL-28B/IFN-lambda 3 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-28B/IFN-lambda 3

Human/mouse IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3), and human IL-29 (IFN-lambda 1) are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11 - 13% amino acid (aa) sequence identity) and type I IFN family (15 - 19% aa sequence identity). These cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Mouse IL-28 shares 61%, 62%, and 52% aa identity with human IL-28A, IL-28B, and IL-29, respectively.

Long Name

Interleukin 28B

Alternate Names

IFN-lambda 3

Gene Symbol

IFNL3

Additional IL-28B/IFN-lambda 3 Products

Product Documents for Human IL-28B/IFN-lambda 3 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human IL-28B/IFN-lambda 3 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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