Human IL-33 ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84818
Key Product Details
Sample Type & Volume Required Per Well
Cell culture supernatant, serum, plasma (citrate, heparin), buffered solution (50 uL)
Sensitivity
0.2 pg/mL
Assay Range
7.8 - 500 pg/mL
Product Specifications
Assay Type
Sandwich ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Human
Specificity
The assay detects both natural and recombinant human IL-33. The cross-reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-33 positive sample. No cross reactivity or interference was detected, notably not with IL-1 alpha, IL-1 beta, and IL-18.
Description
The human IL-33 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-33
Precision
Intra-Assay Precision (Precision within an assay) CV% < 4.7%
Inter-Assay Precision (Precision between assays) CV% < 6.9%
Recovery for Human IL-33 ELISA Kit (Colorimetric)
Recovery
99%
Scientific Data Images for Human IL-33 ELISA Kit (Colorimetric)
Sandwich ELISA: IL-33 ELISA Kit (Colorimetric) [NBP1-84818] - Representative standard curve for human IL-33 ELISA. Human IL-33 was diluted in serial 2-fold steps in CalibratorDiluent. Do not use this standard curve to derive test results. A standard curve must be run for each group ofmicrowell strips assayed.
Kit Contents for Human IL-33 ELISA Kit (Colorimetric)
- Adhesive Films
- Aluminium pouch(es) with a Microwell Plate coated with polyclonal polyclonal antibody to human IL-33
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Biotin-Conjugate anti-human IL-33 polyclonal polyclonal antibody
- Calibrator Diluent
- Conjugate Diluent
- Human IL-33 Standard lyophilized, 500 pg/ml upon reconstitution
- Sample Diluent
- Stop Solution (1M Phosphoric acid)
- Streptavidin-HRP
- Substrate Solution (tetramethyl-benzidine)
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: IL-33
Long Name
Interleukin 33
Alternate Names
C9orf26, DVS27, IL33, NF-HEV
Gene Symbol
IL33
Additional IL-33 Products
Product Documents for Human IL-33 ELISA Kit (Colorimetric)
Product Specific Notices for Human IL-33 ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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