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IL-36 gamma/IL-1F9 Antibody Pair

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-78860

Novus Biologicals, part of Bio-Techne
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NBP2-78860

Key Product Details

Assay Type

Sandwich ELISA

Assay Range

3.9 - 250 pg/ml

Sample Type

Cell Culture Supernatant, Serum (100 ul)

Sensitivity

1 pg/ml

Reactivity

Human

Product Specifications

Description

Sandwich ELISA for the quantitative determination of Human IL-36 gamma/IL-1F9

Sample Volume Required

100 ul

Conjugate

Unconjugated

Application Notes

1. Dilute the desired amount of Coating Antibody (COAT) (1 mg/ml) (mouse monoclonal antibody) to 2 ug/ml in PBS without carrier protein and use it fresh.
2. Dilute the desired amount of Detection Antibody (DET) (0.1 mg/ml) (mouse monoclonal antibody) to 0.1 ug/ml in ELISA Buffer and use it fresh.
3. Reconstitute the Streptavidin-HRP (HRP) with 100 ul ELISA Buffer. After reconstitution, prepare aliquots and store the reconstituted HRP at -20C. Avoid freeze/thaw cycles. Dilute the reconstituted Streptavidin-HRP to the working concentration by adding 10 ul in 10 ml of ELISA Buffer (1:1000).
4. Reconstitute the Standard Protein (STD) (recombinant human IL-36 gamma/IL-1F9) with 100 ul ELISA Buffer to obtain a concentration of 10 ug/ml. After reconstitution, prepare aliquots and store the reconstituted standard at -20C. Avoid freeze/ thaw cycles. A standard curve using 2-fold serial dilutions in ELISA Buffer is recommended. Suggested standard points are 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml, 15.6 pg/ml, 7.8 pg/ml, 3.9 pg/ml and 0 pg/ml.

Kit Contents for IL-36 gamma/IL-1F9 Antibody Pair

  • 1 vial coating antibody (120 ul) [COAT]
  • 1 vial detection antibody (60 ul) [DET]
  • 1 vial standard protein (1 ug) (lyophilized) [STD]
  • 1 vial streptavidin-HRP (10 ug) (lyophilized) [STREP]

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-36 gamma/IL-1F9

Interleukin 36 gamma (IL-36 gamma), also known as IL-1F9 and IL-1H1 is a member of the IL-1 family. IL-36 gamma is secreted when transfected into 293-T cells. Cells reported to express IL-36 gamma include Langerhans cells, keratinocytes/stratified squamos epithelium, chief cells, and parietal cells. The receptor for IL-36 gamma is reported to be a combination of IL-1 R6/IL-1 R rp2 and IL-1 R3/IL-1 R AcP. Recombinant IL-36 gamma has been shown to activate pathways involving NF-kappa B and MAPK in an IL-1 R6/IL-1 R rp2-dependent manner.

Long Name

Interleukin 36 gamma/Interleukin 1 Family 9

Alternate Names

IL-1 H1, IL-1rp2, IL1F9, IL36 gamma, IL36G

Gene Symbol

IL36G

Additional IL-36 gamma/IL-1F9 Products

Product Documents for IL-36 gamma/IL-1F9 Antibody Pair

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IL-36 gamma/IL-1F9 Antibody Pair

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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