Mouse IL-5 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92672

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-92672
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum (50 uL)

Sensitivity

3.3 pg/mL

Assay Range

7.8 - 500 pg/mL

View all IL-5 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

The interference of circulating factors of the immune systemwas evaluated by spiking these proteins at physiologically relevant concentrations into a mouse IL-5 positive serum. There was no crossreactivity detected.

Description

The mouse IL-5 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-5.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.0%

Inter-Assay Precision (Precision between assays) CV% < 10%

Scientific Data Images for Mouse IL-5 ELISA Kit (Colorimetric)

ELISA: Mouse IL-5 ELISA Kit (Colorimetric) [NBP1-92672]

ELISA: Mouse IL-5 ELISA Kit (Colorimetric) [NBP1-92672]

ELISA: IL-5 ELISA Kit [Biotin] [NBP1-92672] - Standard curve

Kit Contents for Mouse IL-5 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-5
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-mouse IL-5 monoclonal antibody
  • Mouse IL-5 Standard lyophilized, 1000 pg/ml upon reconstitution
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-5

Mouse IL-5 has been described as T-cell replacing factor and as B-cell growth factor II based on its induction of proliferation and Ig secretion by activated B cells. Several other activities have furthermore been attributed to this cytokine, including induction of growth and differentiation of eosinophils from bone marrow stew cells, induction of mIL-2R expression on both normal splenic and activated B cells and enhancements of IgA secretion by LPS-stimulated B-cells. mIL-5 has been shown to be produced exclusively by the TH2 subset of mouse TH clones. The spectrum of biological activities of mIL-5, on B cells as well as other cell types suggests that mIL-5 plays an important functional role in the activities of TH2 cells. mIL-5 is a heavily glycosylated glycoprotein.

Long Name

Interleukin 5

Alternate Names

BCDF mu, BCGFII, EDF, Eo-CSF, IL5, TRF

Gene Symbol

IL5

Additional IL-5 Products

Product Documents for Mouse IL-5 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse IL-5 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.