Human IL-5R alpha/CD125 - Ready-To-Use ELISA Kit (Colorimetric)

Name: Human IL-5R alpha/CD125 - Ready-To-Use ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-39626

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-39626

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

24.6 pg/mL (example only; lot dependent)

Assay Range

78.1 - 5000 pg/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human IL-5R alpha/CD125 - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human IL-5R alpha/CD125 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39626]

ELISA: Human IL-5R alpha/CD125 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39626] - Standard Curve Reference

Kit Contents for Human IL-5R alpha/CD125 - Ready-To-Use ELISA Kit (Colorimetric)

Detection Solution A
Detection Solution B
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Standard Diluent
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-5 R alpha/CD125

The protein encoded by the IL5RA gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Six alternatively spliced transcript variants encoding three distinct isoforms have been reported. (provided by RefSeq)

Long Name

Interleukin 5 Receptor alpha

Alternate Names

CD125, IL-5Ra, IL5R alpha, IL5RA

Gene Symbol

IL5RA

Additional IL-5 R alpha/CD125 Products

Product Documents for Human IL-5R alpha/CD125 - Ready-To-Use ELISA Kit (Colorimetric)

Product Datasheets

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COA

Product Specific Notices for Human IL-5R alpha/CD125 - Ready-To-Use ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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