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Human INSL3 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81140

Novus Biologicals, part of Bio-Techne
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NBP2-81140

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.09 ng/mL

Assay Range

0.16 - 10 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human INSL3 in samples. No significant cross-reactivity or interference between Human INSL3 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.18%

Inter-Assay Precision (Precision between assays) CV% < 5.48%

Recovery for Human INSL3 ELISA Kit (Colorimetric)

Recovery

92-109%

Linearity

Human INSL3 ELISA Kit (Colorimetric)

Scientific Data Images for Human INSL3 ELISA Kit (Colorimetric)

Human INSL3 ELISA Kit (Colorimetric)

ELISA: Human INSL3 ELISA Kit (Colorimetric) [NBP2-81140] -

ELISA: Human INSL3 ELISA Kit (Colorimetric) [NBP2-81140] - Standard Curve Reference

Kit Contents for Human INSL3 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: INSL3

Insulin-like 3 (INSL3), a member of the insulin-like hormone superfamily, is specifically expressed in Leydig cells of the fetal and postnatal testis and in theca cells of the postnatal ovary. It is synthesized as a 131-amino acid preproprotein, which contains a 24-amino acid signal peptide. The human INSL3 gene is assigned to bands p13.2-p12 of the short arm of chromosome 19 with the similar organization to that of insulin and relaxin. INSL3 induces gubernaculum development in an androgen-independent way, while androgen-mediated regression of the CSL occurs independently from Insl3. Moreover, INSL3 is a ligand for LGR8 and INSL3-LGR8 mutations are believed to be associated with human cryptorchidism.

Long Name

Insulin-like 3

Alternate Names

Ley I-L, RLF, RLNL

Gene Symbol

INSL3

Additional INSL3 Products

Product Documents for Human INSL3 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human INSL3 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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