Rat KISS1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76460

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

46.88 pg/mL

Assay Range

78.13 - 5000 pg/mL

View all Metastin/KiSS1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat KISS1 in samples. No significant cross-reactivity or interference between Rat KISS1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.93%

Inter-Assay Precision (Precision between assays) CV% < 4.98%

Recovery for Rat KISS1 ELISA Kit (Colorimetric)

Recovery

86-103%

Linearity

Rat KISS1 ELISA Kit (Colorimetric)

Scientific Data Images for Rat KISS1 ELISA Kit (Colorimetric)

Rat KISS1 ELISA Kit (Colorimetric)

ELISA: Rat KISS1 ELISA Kit (Colorimetric) [NBP2-76460] -

ELISA: Rat KISS1 ELISA Kit (Colorimetric) [NBP2-76460] - Standard Curve Reference

Kit Contents for Rat KISS1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Metastin/KiSS1

KISS1 gene encodes metastin, also known as kisspeptin or KiSS-1 peptide, which binds to a G protein-coupled receptor GPR54. KISS1 is dominantly expressed in brain (e.g. anteroventral periventricular nucleus, AVPV arcuate nucleus, ARC), placenta, and pancreas, etc. Metastin plays an important role in regulating the GnRH secretion in brain and MMP-9 expression in placenta, which is associated with development of puberty and sex maturation (e.g. Idiopathic hypogonadotropic hypogonadism, IHH) as well as placentation (e.g. pre-eclampsia), respectively. Metastin was originally discovered as a tumor metastasis suppressor (e.g. melanoma, breast cancer).

Long Name

KiSS-1 Metastasis-suppressor

Alternate Names

KiSS1, Kisspeptin-1

Gene Symbol

KISS1

Additional Metastin/KiSS1 Products

Product Documents for Rat KISS1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat KISS1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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