Human Ku80/XRCC5 ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38918
Key Product Details
Sample Type & Volume Required Per Well
Tissue homogenates and other biological fluids (100 uL)
Sensitivity
0.057 ng/mL (example only; lot dependent)
Assay Range
0.156 - 10 ng/mL (example only; lot dependent)
Product Specifications
Assay Type
Sandwich ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Human
Description
Assay Length: 4.5 hours
Precision
Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)
Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)
Scientific Data Images for Human Ku80/XRCC5 ELISA Kit (Colorimetric)
ELISA: Human Ku80/XRCC5 ELISA Kit (Colorimetric) [NBP3-38918]
ELISA: Human Ku80/XRCC5 ELISA Kit (Colorimetric) [NBP3-38918] - Standard Curve ReferenceKit Contents for Human Ku80/XRCC5 ELISA Kit (Colorimetric)
- Detection Reagent A
- Detection Reagent B
- Diluent Buffer
- Instruction manual
- Plate sealer for 96 wells
- Pre-coated 96T strip plate
- Standard
- Stop Solution
- TMB Substrate
- Wash Buffer (30 x concentrate)
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: Ku80/XRCC5
As one half of the heterodimer complex Ku, Ku80 is required for proper maintenance of the telomeric C strand. Ku80 also plays a role in DNA repair as an essential subunit of DNA-dependent protein kinase (DNA-PK) that phosphorylates certain transcription factors (Sp1, Oct-1 and p53). The heterodimer Ku binds double stranded DNA breaks for non-homologous end joining repair. Ku mutants are defective in T-DNA integration and over-expression confers increased resistance to DNA damage agents and increased susceptibility to T-DNA transformation.
Long Name
Lupus Ku Autoantigen Protein 86
Alternate Names
CTC85, G22P2, KARP1, Ku86, KUB2, NFIV, XRCC5
Gene Symbol
XRCC5
Additional Ku80/XRCC5 Products
Product Documents for Human Ku80/XRCC5 ELISA Kit (Colorimetric)
Product Specific Notices for Human Ku80/XRCC5 ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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