Bovine Lactoferrin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12185

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Milk

Assay Range

0.69 - 500 ng/mL

View all Lactoferrin ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Bovine

Description

Detection method: Biotinylated-Streptavidin-HRP

Scientific Data Images for Bovine Lactoferrin ELISA Kit (Colorimetric)

ELISA: Bovine Lactoferrin ELISA Kit (Colorimetric) [NBP3-12185]

ELISA: Bovine Lactoferrin ELISA Kit (Colorimetric) [NBP3-12185]

ELISA: Bovine Lactoferrin ELISA Kit [NBP3-12185] - Typical standard curve.

Kit Contents for Bovine Lactoferrin ELISA Kit (Colorimetric)

  • Bovine Lactoferrin Detection Antibody - 12 mL
  • Bovine Lactoferrin Pre-Coated 96-well Strip Plate - 1 EA
  • Bovine Lactoferrin Standard (250 ng / vial) - 2 EA
  • Dilution Buffer C (20X) - 25 mL
  • HRP Solution C - 12 mL
  • Sealing Tape - 6 sheets
  • Stop Solution - 12 mL
  • TMB Substrate - 12 mL
  • Wash Buffer (20X) - 50 mL

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4°C.

Background: Lactoferrin

Lactoferrin is an iron binding glycoprotein with an approximate molecular weight of 80 kDa. The protein has two iron binding domains each housing one Fe3+ and the synergistic CO32- ion. The crystal structure form of human lactoferrin at 2.2A resolution exhibits 5330 protein atoms, 2Fe2+, 2CO32- and 98 carbohydrate atoms. Lactoferrin is absorbed from intestine by apical side of the membrane and localized to the nuclei. Intravenous infusion of lactoferrin is protective against lethal doses of E coli and induce bacterimia by a mechanism that downregulates neutrophil TNF alfa secretion. Recombinant human lactoferrin (rhLF), expressed and extracted from rice seed, is being evaluated for use as a dietary supplement to treat iron deficiency and/or iron deficiency induced anemia. Lactoferrin has been shown to have a role in the immune system and in early development of the embryo. A specific receptor for lactoferrin binding has been implicated in the human fetal intestine. Early embryonic localisation of lactoferrin by IHC has suggested its presence in various tissues including intestinal epitheliuem, kiney, and various regions of the brain.

Alternate Names

GIG12, HEL110, HLF2, Kaliocin-1, Lactoferricin, Lactoferroxin, Lactotransferrin, LF, LTF, Talalactoferrin

Gene Symbol

LTF

Additional Lactoferrin Products

Product Documents for Bovine Lactoferrin ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Bovine Lactoferrin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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