MCAM/CD146 Antibody Pair [HRP]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79445

Novus Biologicals, part of Bio-Techne
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Key Product Details

Assay Type

Sandwich ELISA

Assay Range

11-700 pg/ml (example only; lot dependent)

Sensitivity

31.25 pg/ml (example only; lot dependent)

Reactivity

Human

View all MCAM/CD146 ELISA Kits »

Product Specifications

Description

Solid Phase sandwich ELISA for the quantitative determination of Human MCAM/CD146.

Sample Volume Required

100 ul

Conjugate

HRP

Scientific Data Images for MCAM/CD146 Antibody Pair [HRP]

Sandwich ELISA: MCAM/CD146 Antibody Pair [HRP] [NBP2-79445] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Kit Contents for MCAM/CD146 Antibody Pair [HRP]

  • Mouse Monoclonal Capture Antibody
  • Rabbit Monoclonal Detection Antibody (HRP-conjugated): (Catalog # NBP2-89276)
  • Standard

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: MCAM/CD146

CD146 belongs to the immunoglobulin supergene family with five immunoglobulin like domains (V-V-C2-C2-C2), a transmembrane region and a 63 residue cytoplasmic tail. The protein is a membrane glycoprotein that functions as a Ca2+ independent cell adhesion molecule involved in heterophilic cell to cell interactions. CD146 has a molecular size of 130 kDa in its reduced form (118 kDa unreduced), and N linked glycosylation accounts for fifty percent of the apparent molecular weight. In some cells the molecule carries a sulfate 3 glucuronyl moiety. Expression of the molecule was shown in a relatively limited spectrum of normal human tissues (endothelium, smooth muscle, and subpopulations of activated T cells) and malignant neoplasm (melanoma and breast carcinoma). The lineage specific expression pattern of CD146 can be useful in the differential diagnosis of certain lesions including melanomas and various types of gestational trophoblastic lesions. CD146 expression can promote tumor progression in human melanoma, through enhanced interaction between melanoma cells and endothelial cells. However, in breast carcinoma, CD146 may act as a tumor suppressor. Over expression of CD146 in breast carcinoma cells results in a more cohesive cell growth and in the formation of smaller tumors in nude mice. During implantation and placentation, CD146 is expressed by the intermediate trophoblast in the placental site and binds to its putative receptor in uterine smooth muscle cells thus limiting trophoblastic invasion in the myometrium. Monoclonal antibody specific for CD146 is an important tool for the identification and isolation of cells expressing CD146.

Long Name

Melanoma Cell Adhesion Molecule

Alternate Names

CD146, L-Gicerin, MUC18

Gene Symbol

MCAM

Additional MCAM/CD146 Products

Product Documents for MCAM/CD146 Antibody Pair [HRP]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MCAM/CD146 Antibody Pair [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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