Melatonin ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62160
Key Product Details
Sample Type & Volume Required Per Well
Saliva, serum, plasma, fruit samples (100 uL)
Sensitivity
0.162 ng/ml
Assay Range
0.08-50 ng/ml
Product Specifications
Assay Type
Competitive ELISA
Kit Type
ELISA Kit (Colorimetric)
Precision
Intra-Assay Precision (Precision within an assay) Low Concentration %CV - 13.74% High Concentration %CV - 4.31%
Inter-Assay Precision (Precision between assays) Low Concentration %CV - 17.54% High Concentration %CV - 7.36%
Recovery for Melatonin ELISA Kit (Colorimetric)
Recovery
Human Serum
Low - 124%
Medium - 83%
High - 102%
Human EDTA Plasma Low - 112.2%
Medium - 90%
High - 101%
Rat Serum Low - 112.4%
Medium - 99.6%
High - 89.7%
Mouse Serum Low - 115.6%
Medium - 105.8%
Medium - 83%
High - 102%
Human EDTA Plasma Low - 112.2%
Medium - 90%
High - 101%
Rat Serum Low - 112.4%
Medium - 99.6%
High - 89.7%
Mouse Serum Low - 115.6%
Medium - 105.8%
Scientific Data Images for Melatonin ELISA Kit (Colorimetric)
![ELISA: Melatonin ELISA Kit (Colorimetric) [NBP2-62160]](https://resources.bio-techne.com/images/products/Melatonin-ELISA-Kit-Colorimetric-ELISA-NBP2-62160-img0001.jpg "ELISA: Melatonin ELISA Kit (Colorimetric) [NBP2-62160]")
ELISA: Melatonin ELISA Kit (Colorimetric) [NBP2-62160]
ELISA: Melatonin ELISA Kit (Colorimetric) [NBP2-62160] - Standard Reference Curve![ELISA: Melatonin ELISA Kit (Colorimetric) [NBP2-62160]](https://resources.bio-techne.com/images/products/Melatonin-ELISA-Kit-Colorimetric-ELISA-NBP2-62160-img0002.jpg "ELISA: Melatonin ELISA Kit (Colorimetric) [NBP2-62160]")
ELISA: Melatonin ELISA Kit (Colorimetric) [NBP2-62160]
ELISA: Melatonin ELISA Kit (Colorimetric) [NBP2-62160] - To assess parallelism, human serum and EDTA plasma samples,human saliva, porcine along with other matrix samples wereserially diluted in 1X Stabilizer. The samples were then run in theassay. The melatonin concentration in each sample wasdetermined from the standard curve. Concentrations were plottedas a function of sample dilution. Parallelism of the curvesdemonstrates that the standard binding characteristics are similarenough to allow the accurate determination of native melatoninlevels in diluted samples matrices of animal and plant origin.Kit Contents for Melatonin ELISA Kit (Colorimetric)
- and Stop Solution
- Antibody
- Assay Buffer 10
- Conjugate
- Goat anti-mouse IgG Microtiter Plate
- Standard
- TMB Substrate
- Tracer
- Wash Buffer Concentrate
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: Melatonin
Additional Melatonin Products
Product Documents for Melatonin ELISA Kit (Colorimetric)
Product Specific Notices for Melatonin ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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