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Porcine MIS/AMH ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-42306

Novus Biologicals, part of Bio-Techne
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NBP3-42306

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids (50 uL)

Sensitivity

0.75 ng/mL

Assay Range

1.88 - 30 ng/mL

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Porcine

Description

Assay time: 2 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10

Inter-Assay Precision (Precision between assays) %CV < 12

Kit Contents for Porcine MIS/AMH ELISA Kit (Colorimetric)

  • Assay Diluent A
  • Assay Diluent B
  • Detection Reagent A
  • Detection Reagent B
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated ready to use 96-well strip plate
  • Standard
  • Standard Diluent
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: MIS/AMH

Müllerian inhibiting substance (MIS), also named anti-Müllerian hormone (AMH), is a tissue-specific TGF-beta superfamily growth factor. Its expression is restricted to Sertoli cells of fetal and postnatal testis, and to granulosa cells of postnatal ovary. MIS is synthesized and secreted as a disulfide-linked homodimeric propeptide. Proteolytic cleavage is required to separate the N-terminal proregion from the C-terminal bioactive peptide. Both fragments remain associated in a non-covalent complex. The C-terminal sequence forms a cysteine knot via seven canonical cysteine residues found in TGF-beta superfamily members.

Long Name

Mullerian-inhibiting Substance/Anti-Mullerian Hormone

Alternate Names

AMH

Gene Symbol

AMH

Additional MIS/AMH Products

Product Documents for Porcine MIS/AMH ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Porcine MIS/AMH ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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