Rat MMP-11 ELISA Kit (Colorimetric)

Name: Rat MMP-11 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-06936

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06936

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 ul)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat MMP-11 in samples. No significant cross-reactivity or interference between Rat MMP-11 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 5.61

Inter-Assay Precision (Precision between assays) 4.67

Recovery for Rat MMP-11 ELISA Kit (Colorimetric)

Recovery

93-106 %

Linearity

Scientific Data Images for Rat MMP-11 ELISA Kit (Colorimetric)

ELISA: Rat MMP-11 ELISA Kit (Colorimetric) [NBP3-06936] -

ELISA: Rat MMP-11 ELISA Kit (Colorimetric) [NBP3-06936] - Standard Curve Reference

Kit Contents for Rat MMP-11 ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: MMP-11

The matrix metalloproteinases are a tightly regulated family of enzymes that degrade extracellular matrix and basement membrane components. Recent evidence suggests that these proteases and their specific inhibitors play important roles in normal developmental processes and in pathological conditions (1). MMP-11 (stromelysin-3), a new member of the matrix metalloproteinase family, is expressed in tissues undergoing the active remodeling associated with embryonic development, wound healing and tumor invasion. But like all other members of the matrix metalloproteinase gene family, stromelysin-3 is synthesized as an inactive precursor that must be processed to its mature form in order to express enzymic activity (2). MMP-11, like an inflammatory mediator, may exert a chemotactic influence on macrophages which aggregate in the mesangium; MMP-11 is not likely to have a parallel mitogenic or antifibrotic effect in diseased glomeruli (3).

Long Name

Matrix Metalloproteinase 11

Alternate Names

MMP11

Gene Symbol