Human MMP-25/MT6-MMP ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06943

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 ul)

Sensitivity

0.09 ng/mL

Assay Range

0.16 - 10 ng/mL

View all MMP-25/MT6-MMP ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human MMP-25/MT6-MMP in samples. No significant cross-reactivity or interference between Human MMP-25/MT6-MMP and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 4.52

Inter-Assay Precision (Precision between assays) 5.15

Recovery for Human MMP-25/MT6-MMP ELISA Kit (Colorimetric)

Recovery

88-102 %

Linearity

Human MMP-25/MT6-MMP ELISA Kit (Colorimetric)

Scientific Data Images for Human MMP-25/MT6-MMP ELISA Kit (Colorimetric)

Human MMP-25/MT6-MMP ELISA Kit (Colorimetric)

ELISA: Human MMP-25/MT6-MMP ELISA Kit (Colorimetric) [NBP3-06943] -

ELISA: Human MMP-25/MT6-MMP ELISA Kit (Colorimetric) [NBP3-06943] - Standard Curve Reference

Kit Contents for Human MMP-25/MT6-MMP ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: MMP-25/MT6-MMP

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling. They are also involved in disease processes, such as metastasis and arthritis. Most MMPs are secreted as inactive proproteins which are then activated when cleaved by extracellular proteinases. The protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily, and is attached to the plasma membrane via a glycosylphosphatidyl inositol anchor. The encoded protein is thought to inactivate alpha-1 proteinase inhibitor, a major tissue protectant against proteolytic enzymes released by activated neutrophils, facilitating the transendothelial migration of neutrophils to inflammatory sites in response to bacterial infection or inflammation. The encoded protein may also play a role in tumor invasion and metastasis through activation of MMP2. The gene has previously been referred to as MMP20 but has been renamed MMP25.

Long Name

Matrix Metalloproteinase 25/Membrane Type 6 MMP

Alternate Names

Leukolysin, MMP25, MT6-MMP

Gene Symbol

MMP25

Additional MMP-25/MT6-MMP Products

Product Documents for Human MMP-25/MT6-MMP ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human MMP-25/MT6-MMP ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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