Mouse MFG-E8 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MFGE80
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 µL), Serum (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.02 ng/mL
Assay Range
0.125-8 ng/mL
Product Summary for Mouse MFG-E8 Quantikine ELISA Kit
The Quantikine Mouse MFG-E8 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse MFG-E8 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse MFG-E8 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse MFG-E8 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse MFG-E8.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse MFG-E8
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 0.506 | 0.936 | 3.89 | 0.509 | 0.910 | 3.78 |
| Standard Deviation | 0.020 | 0.042 | 0.116 | 0.047 | 0.071 | 0.322 |
| CV% | 4.0 | 4.5 | 3.0 | 9.2 | 7.8 | 8.5 |
Recovery for Mouse MFG-E8 Quantikine ELISA Kit
The recovery of MFG-E8 spiked into various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 99 | 90-104 |
| Heparin Plasma (n=4) | 94 | 90-98 |
| Serum (n=4) | 101 | 97-104 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse MFG-E8 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse MFG-E8 Quantikine ELISA Kit
Mouse MFG-E8 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MFG-E8
Long Name
Milk Fat Globule EGF Factor 8
Alternate Names
BA46, Breast epithelial antigen BA46, Lactahedrin, Medin, MFGE8, SED1
Gene Symbol
MFGE8
Additional MFG-E8 Products
Product Documents for Mouse MFG-E8 Quantikine ELISA Kit
Product Specific Notices for Mouse MFG-E8 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.