Mouse PD-1 DuoSet ELISA

Key Product Details

Product Specifications

Kit Contents for Mouse PD-1 DuoSet ELISA

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates):(Catalog #DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS:(Catalog #DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer:(Catalog #WA126), or equivalent

Reagent Diluent:1% BSA in PBS, pH 7.2-7.4, 0.2 m filtered (R&D Systems Catalog #DY995). Quality of BSA is critical (see Technical Hints).

Blocking Buffer:1% BSA in PBS, pH 7.2-7.4, 0.2 m filtered (R&D Systems Catalog #DY995). Quality of BSA is critical (see Technical Hints).

Substrate Solution:1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog #DY999)

Stop Solution:2 N H₂SO₄(Catalog #DY994)

Microplates: R&D Systems (Catalog #DY990), or equivalent

Plate Sealers:ELISA Plate Sealers (Catalog #DY992), or equivalent

Preparation and Storage

Background: PD-1

Additional PD-1 Products

• All Products for PD-1
• PD-1 cDNA Clones
• PD-1 ELISA Kits
• PD-1 Lysates
• PD-1 Primary Antibodies
• PD-1 Proteins and Enzymes