Human Myoglobin ELISA Kit (Chemiluminescence)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68064

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.38 ng/mL

Assay Range

0.63-40 ng/mL

View all Myoglobin ELISA Kits »

Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Human

Specificity

This kit recognizes Human MYO in samples. No significant cross-reactivity or interference between Human MYO and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 10.26%

Inter-Assay Precision (Precision between assays) CV% < 11.08%

Recovery for Human Myoglobin ELISA Kit (Chemiluminescence)

Recovery

88-113%

Linearity

Human Myoglobin ELISA Kit (Chemiluminescence)

Scientific Data Images for Human Myoglobin ELISA Kit (Chemiluminescence)

Human Myoglobin ELISA Kit (Chemiluminescence)

ELISA: Human Myoglobin ELISA Kit (Chemiluminescence) [NBP2-68064] -

ELISA: Human Myoglobin ELISA Kit (Chemiluminescence) [NBP2-68064] - Standard Curve Reference

Kit Contents for Human Myoglobin ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Myoglobin

Myoglobin is a small heme containing protein (153 amino acid residues, molecular weight (w/o heme) 17053 Da and theoretical pI=7.29) responsible for the oxygen deposition in muscle tissues. Only one form of myoglobin is expressed in cardiac and skeletal muscles. Myoglobin is known as a marker of myocardial damage and it has been used for more than three decades. Nowadays it still is very commonly used in clinical practice as an early marker of AMI. It appears in patient's blood 1 to 3 hours after onset of the symptoms, reaching peak level within 8 to 12 hours. Myoglobin is not so cardiac specific as cTnI or cTnT. Because of high myoglobin concentration in skeletal muscle tissue, even minor skeletal muscle injury results in the significant increase of myoglobin concentration in blood. Thus myoglobin is used together with cTnI or cTnT in clinical practise for better specificity in AMI diagnosis.

Alternate Names

MB

Gene Symbol

MB

Additional Myoglobin Products

Product Documents for Human Myoglobin ELISA Kit (Chemiluminescence)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Myoglobin ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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