Rat nNOS ELISA Kit (Colorimetric)

Name: Rat nNOS ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-80254

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80254

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 ul)

Sensitivity

0.094 ng/mL

Assay Range

0.156-10 ng/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat NOS1/nNOS in samples. No significant cross-reactivity or interference between Rat NOS1/nNOS and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.87%

Inter-Assay Precision (Precision between assays) CV% < 4.97%

Recovery for Rat nNOS ELISA Kit (Colorimetric)

Recovery

85-101%

Linearity

Scientific Data Images for Rat nNOS ELISA Kit (Colorimetric)

ELISA: Rat nNOS ELISA Kit (Colorimetric) [NBP2-80254] -

ELISA: Rat nNOS ELISA Kit (Colorimetric) [NBP2-80254] - Standard Curve Reference

Kit Contents for Rat nNOS ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100X)
Concentrated HRP Conjugate (100X)
Concentrated Wash Buffer (25X)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: nNOS

NOS (nitric oxide synathase) is a mediator of NO (nitric oxide) production, an inorganic gaseous messenger between cells. In cells, NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Three isoforms of NOS has been identified (eNOS, iNOS, and nNOS) with all of them having binding sites for NADPH, FAD, FMN, and tetrahydrobiopterin (1). Specifically, nNOS (Neuronal nitric oxide synthase bNOS, cNOS, Type I) is bound to PSD95 located in the neuronal membrane and at increased level of Ca2+, nNOS is trasnlocated to cytoplasm. In cytoplasm, nNOS is dephosphorylated by calcineurin which initiates NO production (2). A phosphorylation by PKA and PKC on nNOS leads down-regulation of NO production.

Long Name

Neuronal Nitric Oxide Synthase

Alternate Names

NOS1

Gene Symbol