Human Nucleolin - Ready-To-Use ELISA Kit (Colorimetric)

Name: Human Nucleolin - Ready-To-Use ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-39209

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-39209

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.057 ng/mL (example only; lot dependent)

Assay Range

0.156 - 10 ng/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human Nucleolin - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human Nucleolin - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39209]

ELISA: Human Nucleolin - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39209] - Standard Curve Reference

Kit Contents for Human Nucleolin - Ready-To-Use ELISA Kit (Colorimetric)

Detection Solution A
Detection Solution B
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Standard Diluent
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Nucleolin

Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein. It is the major protein in actively dividing cells, whereas the degraded forms are relatively abundant in non-dividing cells. Stability of the nucleolin protein is cell proliferation-dependent. It plays a role in rDNA transcription, organization, and rRNA processing. It may alter DNA organization in response to cell cycle controls. Nucleolin antibodies are commonly used as a mammalian nucleolus marker.

Alternate Names

C23, FLJ45706, FLJ59041, nucleolin, Protein C23

Gene Symbol

NCL

Additional Nucleolin Products

Product Documents for Human Nucleolin - Ready-To-Use ELISA Kit (Colorimetric)

Product Datasheets

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COA

Product Specific Notices for Human Nucleolin - Ready-To-Use ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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