Human Orexin A/Hypocretin-1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80230

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 ul)

Sensitivity

37.5 pg/mL

Assay Range

62.5-4000 pg/mL

View all Orexin A/Hypocretin-1 ELISA Kits »

Product Specifications

Assay Type

Competitive-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human OXA in samples. No significant cross-reactivity or interference between Human OXA and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.71%

Inter-Assay Precision (Precision between assays) CV% < 4.59%

Recovery for Human Orexin A/Hypocretin-1 ELISA Kit (Colorimetric)

Recovery

88-111%

Linearity

Human Orexin A/Hypocretin-1 ELISA Kit (Colorimetric)

Scientific Data Images for Human Orexin A/Hypocretin-1 ELISA Kit (Colorimetric)

Human Orexin A/Hypocretin-1 ELISA Kit (Colorimetric)

ELISA: Human Orexin A/Hypocretin-1 ELISA Kit (Colorimetric) [NBP2-80230] -

ELISA: Human Orexin A/Hypocretin-1 ELISA Kit (Colorimetric) [NBP2-80230] - Standard Curve Reference

Kit Contents for Human Orexin A/Hypocretin-1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100X)
  • Concentrated HRP Conjugate (100X)
  • Concentrated Wash Buffer (25X)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Orexin A/Hypocretin-1

FUNCTION: Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity (By similarity). SUBCELLULAR LOCATION: Endoplasmic reticulum; rough endoplasmic reticulum (By similarity). Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses (By similarity). SIMILARITY: Belongs to the orexin family.

Alternate Names

HCRT, Hcrt1, Hypocretin-1, Hypocretin1

Gene Symbol

HCRT

UniProt

O43612

Additional Orexin A/Hypocretin-1 Products

Product Documents for Human Orexin A/Hypocretin-1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Orexin A/Hypocretin-1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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