Monkey Osteopontin/OPN ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68076

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100uL)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

View all Osteopontin/OPN ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Monkey

Specificity

This kit recognizes Monkey OPN in samples. No significant cross-reactivity or interference between Monkey OPN and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.62%

Inter-Assay Precision (Precision between assays) CV% < 4.33%

Recovery for Monkey Osteopontin/OPN ELISA Kit (Colorimetric)

Recovery

83-110%

Linearity

Monkey Osteopontin/OPN ELISA Kit (Colorimetric)

Scientific Data Images for Monkey Osteopontin/OPN ELISA Kit (Colorimetric)

Monkey Osteopontin/OPN ELISA Kit (Colorimetric)

ELISA: Monkey Osteopontin/OPN ELISA Kit (Colorimetric) [NBP2-68076] -

ELISA: Monkey Osteopontin/OPN ELISA Kit (Colorimetric) [NBP2-68076] - Standard Curve Reference

Kit Contents for Monkey Osteopontin/OPN ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Osteopontin/OPN

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Long Name

Secreted Phosphoprotein 1 [BNSP]

Alternate Names

Eta-1, OPN, Spp1

Gene Symbol

SPP1

Additional Osteopontin/OPN Products

Product Documents for Monkey Osteopontin/OPN ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Monkey Osteopontin/OPN ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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