Human, Mouse, Rat p62/SQSTM1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61300

Novus Biologicals, part of Bio-Techne
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NBP2-61300
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Key Product Details

Sample Type & Volume Required Per Well

cell lysate samples Cited sample type includes in PBMC lysates (100 uL)

Sensitivity

100pg/ml

Assay Range

625 - 40,000pg/ml

View all p62/SQSTM1 ELISA Kits »

Product Specifications

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human, Mouse, Rat

Description

Store all components at 4C, except standard at -80C.

Precision

Intra-Assay Precision (Precision within an assay) Mean 3.9%

Inter-Assay Precision (Precision between assays) Mean 8.4%

Recovery for Human, Mouse, Rat p62/SQSTM1 ELISA Kit (Colorimetric)

Recovery

HeLa Lysate
Low - 125%
Medium - 111%
High - 95%
Jurkat Lysate
Low - 85%
Medium - 97%
High - 30%
3T3 Lysate
Low - 95%
Medium - 75%
High - 80%
C6 Lysate
Low - 76%
Medium - 86%
High - 89%

Scientific Data Images for Human, Mouse, Rat p62/SQSTM1 ELISA Kit (Colorimetric)

ELISA: Human, Mouse, Rat p62/SQSTM1 ELISA Kit (Colorimetric) [NBP2-61300]

ELISA: Human, Mouse, Rat p62/SQSTM1 ELISA Kit (Colorimetric) [NBP2-61300]

ELISA: p62/SQSTM1 ELISA Kit (Colorimetric) [NBP2-61300] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ELISA: Human, Mouse, Rat p62/SQSTM1 ELISA Kit (Colorimetric) [NBP2-61300]

ELISA: Human, Mouse, Rat p62/SQSTM1 ELISA Kit (Colorimetric) [NBP2-61300]

ELISA: p62/SQSTM1 ELISA Kit (Colorimetric) [NBP2-61300] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Kit Contents for Human, Mouse, Rat p62/SQSTM1 ELISA Kit (Colorimetric)

  • Antibody
  • Assay Buffer 13
  • Conjugate
  • Microtiter Plate
  • RIPA Cell Lysis Buffer 2
  • Standard
  • Stop Solution 2
  • TMB Substrate
  • Wash Buffer Concentrate

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: p62/SQSTM1

p62/SQSTM1 (ubiquitin-binding protein p62/Sequestrosome-1) is an intracellular protein (theoretical molecular weight 47.7 kDa) which localizes to the cytoplasm, nucleus, autophagosomes and lysosomes in all tissues (1). Structurally, p62/SQSTM1 consists of multiple domains (e.g., Phox1 and Bem1p-PB1, zinc finger-ZZ, TRAF6 binding domain-TB, Keap1-interacting region-KIR, LC3 interacting region-LIR and ubiquitin-associated domain-UBA) for interaction with various protein targets. Additional domains include nuclear export (NES) and nuclear localization signals (NLS1 and NLS2). p62/SQSTM1 is a multifunctional scaffold protein which forms oligomers with itself or with other proteins through interactions with PB1 domains.

Abnormal function of p62/SQSTM1 is associated with a range of disease states such as neurodegeneration, cancer, and metabolic disorders (2). Mutations in the p62/SQSTM1 sequence have been linked to Paget's disease of the bone, amyotrophic lateral sclerosis, and frontotemporal lobar degeneration. In Parkinson's disease, p62/SQSTM1 has been linked to microglia activation and subsequent neuroinflammation (3). Functionally, p62/SQSTM1 is involved in a broad range of cellular processes such as amino acid sensing by interaction with mTORC1, oxidative stress response through interaction with Keap1, and targeting cargo for autophagy by interacting with ubiquitin labeled proteins (1).

To induce selective autophagy, p62/SQSTM1 forms long oligomers or helical filaments which interact with LC3 and ubiquitin labeled proteins and lead to the initiation of the autophagosome formation (2). p62/SQSTM1 is not only a selective autophagy receptor but also an autophagy substrate, as its engulfed by the autophagosome and degraded by the autophagolysosome. Monitoring LC3 levels is the standard for assessing autophagic flux, however monitoring p62/SQSTM1 levels by Western blot in the presence and absence of autophagy inhibitors (e.g., Chloroquine) is also a common practice (4). Besides its activity as a selective autophagy receptor, p62/SQSTM1 also plays a role as an adaptor in signaling cascades leading to NFkB activation downstream of TNF-R, IL-1 beta R, TrkA and p75NTR. Briefly, for NFkB signaling downstream of the TNF-R activation, p62/SQSTM1 engages RIP1 kinase and PKC iota/lambda through the ZZ and PB1 domains, respectively (5).

References

1.Katsuragi, Y., Ichimura, Y., & Komatsu, M. (2015). P62/SQSTM1 functions as a signaling hub and an autophagy adaptor. FEBS Journal. https://doi.org/10.1111/febs.13540

2. Sanchez-Martin, P., & Komatsu, M. (2018). p62/SQSTM1 - Steering the cell through health and disease. Journal of Cell Science. https://doi.org/10.1242/jcs.222836

3. Yao, L., Zhu, Z., Wu, J., Zhang, Y., Zhang, H., Sun, X., ... Lu, G. (2019). MicroRNA-124 regulates the expression of p62/p38 and promotes autophagy in the inflammatory pathogenesis of Parkinson's disease. The FASEB Journal. https://doi.org/10.1096/fj.201900363r

4 Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., Abeliovich, H., Arozena, A. A., ... Zughaier, S. M. (2016). Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy. https://doi.org/10.1080/15548627.2015.1100356

5. Bitto, A., Lerner, C. A., Nacarelli, T., Crowe, E., Torres, C., & Sell, C. (2014). p62/SQSTM1 at the interface of aging, autophagy, and disease. Age. https://doi.org/10.1007/s11357-014-9626-3

Long Name

Sequestosome 1

Alternate Names

A170, EBIAP, ORCA, OSIL, PDB3, Sequestosome 1, SQSTM1, ZIP3

Gene Symbol

SQSTM1

Additional p62/SQSTM1 Products

Product Documents for Human, Mouse, Rat p62/SQSTM1 ELISA Kit (Colorimetric)

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Certificate of Analysis

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Product Specific Notices for Human, Mouse, Rat p62/SQSTM1 ELISA Kit (Colorimetric)

Store all components at 4C, except standard at -80C.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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