Rat PACAP/ADCYAP1 ELISA Kit (Colorimetric)

Name: Rat PACAP/ADCYAP1 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-78762

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-78762

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

4.69 pg/mL

Assay Range

7.81 - 500 pg/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat PACAP in samples. No significant cross-reactivity or interference between Rat PACAP and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.20%

Inter-Assay Precision (Precision between assays) CV% < 5.45%

Recovery for Rat PACAP/ADCYAP1 ELISA Kit (Colorimetric)

Recovery

91-106%

Linearity

Scientific Data Images for Rat PACAP/ADCYAP1 ELISA Kit (Colorimetric)

ELISA: Rat PACAP/ADCYAP1 ELISA Kit (Colorimetric) [NBP2-78762] -

ELISA: Rat PACAP/ADCYAP1 ELISA Kit (Colorimetric) [NBP2-78762] - Standard Curve Reference

Kit Contents for Rat PACAP/ADCYAP1 ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PACAP/ADCYAP1

ADCYAP1 encodes adenylate cyclase activating polypeptide 1. Mediated by adenylate cyclase activating polypeptide 1 receptors, this polypeptide stimulates adenylate cyclase and subsequently increases the cAMP level in target cells. Adenylate cyclase activating polypeptide 1 is not only a hypophysiotropic hormone, but also functions as a neurotransmitter and neuromodulator. In addition, it plays a role in paracrine and autocrine regulation of certain types of cells. This gene encodes three different mature peptides, including two isotypes, a shorter form and a longer form. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Long Name

Pituitary Adenylate Cyclase-activating Polypeptide

Alternate Names

ADCYAP1

Gene Symbol