Human PAR4 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06971

Novus Biologicals, part of Bio-Techne
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NBP3-06971
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 ul)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

View all PAR4 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human PAR4 in samples. No significant cross-reactivity or interference between Human PAR4 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 5.54

Inter-Assay Precision (Precision between assays) 4.64

Recovery for Human PAR4 ELISA Kit (Colorimetric)

Recovery

86-107 %

Linearity

Human PAR4 ELISA Kit (Colorimetric)

Scientific Data Images for Human PAR4 ELISA Kit (Colorimetric)

Human PAR4 ELISA Kit (Colorimetric)

ELISA: Human PAR4 ELISA Kit (Colorimetric) [NBP3-06971] -

ELISA: Human PAR4 ELISA Kit (Colorimetric) [NBP3-06971] - Standard Curve Reference

Kit Contents for Human PAR4 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PAR4

PAR4, also known as F2RL3 and Coagulation Factor II (Thrombin) Receptor-like 3, is a member of the Protease-activated Receptor (PAR) family of seven transmembrane G protein-coupled receptors that are activated by proteolysis. It is 385 amino acids (aa) in length with a predicted molecular weight of 41.1 kDa. Human PAR4 shares 78% aa sequence identity with the mouse ortholog. PAR4 is widely expressed with highest expression levels being found in the lungs, pancreas, thyroid, testis, and small intestine. It is also observed in platelets, megakaryocytes, and leukocytes. PAR4 is activated by Thrombin, Trypsin, and Cathepsin G. These proteases bind to and cleave the N-terminal extracellular domain of PAR4 to generate a new N-terminus. This new N-terminus then functions as a tethered peptide ligand that binds to and activates the receptor. PAR4 is coupled to G proteins containing the alphaq and alpha12/13 subunits and has been shown to activate the PLC-IP3-Ca2+ and PLC-DAG-PKC signal transduction pathways. PAR4 plays an essential role in hemostasis and thrombosis. Interestingly, PAR4 requires PAR3 as a cofactor to achieve optimal Thrombin-induced platelet activation. PAR4 has also been suggested to be involved in regulation of gastrointestinal motility, modulation of nociceptive responses, and stimulation of epithelial to mesenchymal transition in alveolar epithelial cells. Down-regulation of PAR4 expression occurs frequently in gastric cancers and is associated with an aggressive progression of gastric cancer.

Long Name

Protease-Activated Receptor 4

Alternate Names

F2RL3, Protease-Activated Receptor 4, Thrombin Receptor-like 3

Gene Symbol

F2RL3

Additional PAR4 Products

Product Documents for Human PAR4 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human PAR4 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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