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Rat PCK1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81241

Novus Biologicals, part of Bio-Techne
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NBP2-81241

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.09 ng/mL

Assay Range

0.16 - 10 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat PCK1 in samples. No significant cross-reactivity or interference between Rat PCK1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.08%

Inter-Assay Precision (Precision between assays) CV% < 5.2%

Recovery for Rat PCK1 ELISA Kit (Colorimetric)

Recovery

89-108%

Linearity

Rat PCK1 ELISA Kit (Colorimetric)

Scientific Data Images for Rat PCK1 ELISA Kit (Colorimetric)

Rat PCK1 ELISA Kit (Colorimetric)

ELISA: Rat PCK1 ELISA Kit (Colorimetric) [NBP2-81241] -

ELISA: Rat PCK1 ELISA Kit (Colorimetric) [NBP2-81241] - Standard Curve Reference

Kit Contents for Rat PCK1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PCK1

The PCK1 gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expressio

Long Name

Phosphoenolpyruvate Carboxykinase 1

Alternate Names

PEPCK-C, PEPCK1

Gene Symbol

PCK1

Additional PCK1 Products

Product Documents for Rat PCK1 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat PCK1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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