Human PCNA ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18219
Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma and cell culture supernates (100 ul)
Sensitivity
0.055 ng/ml
Assay Range
0.39-25 ng/ml
Product Specifications
Assay Type
Sandwich ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Human
Description
The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human PCNA in the sample, this Human PCNA ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human PCNA concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.
Precision
Intra-Assay Precision (Precision within an assay) %CV < 10
Inter-Assay Precision (Precision between assays) %CV < 15
Recovery for Human PCNA ELISA Kit (Colorimetric)
Recovery
87-99%
Scientific Data Images for Human PCNA ELISA Kit (Colorimetric)
![ELISA: Human PCNA ELISA Kit (Colorimetric) [NBP3-18219]](https://resources.bio-techne.com/images/products/Human-PCNA-ELISA-Kit-Colorimetric-ELISA-NBP3-18219-img0001.jpg "ELISA: Human PCNA ELISA Kit (Colorimetric) [NBP3-18219]")
ELISA: Human PCNA ELISA Kit (Colorimetric) [NBP3-18219]
ELISA: Human PCNA ELISA Kit (Colorimetric) [NBP3-18219] - Standard Curve ReferenceKit Contents for Human PCNA ELISA Kit (Colorimetric)
- Balance Solution
- Enzyme Conjugate
- Microtiter Plate
- Standards
- Stop Solution
- Substrate A
- Substrate B
- Wash Solution
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: PCNA
Long Name
Proliferating Cell Nuclear Antigen
Alternate Names
Cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen
Gene Symbol
PCNA
Additional PCNA Products
Product Documents for Human PCNA ELISA Kit (Colorimetric)
Product Specific Notices for Human PCNA ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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