Human Perilipin-3/TIP47 ELISA Kit (Colorimetric)

Name: Human Perilipin-3/TIP47 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-39294

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-39294

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates and other biological fluids (100 uL)

Sensitivity

0.059 ng/mL (example only; lot dependent)

Assay Range

0.156 - 10 ng/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human Perilipin-3/TIP47 ELISA Kit (Colorimetric)

ELISA: Human Perilipin-3/TIP47 ELISA Kit (Colorimetric) [NBP3-39294]

ELISA: Human Perilipin-3/TIP47 ELISA Kit (Colorimetric) [NBP3-39294] - Standard Curve Reference

Kit Contents for Human Perilipin-3/TIP47 ELISA Kit (Colorimetric)

Detection Reagent A
Detection Reagent B
Diluent Buffer
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Perilipin-3

TIP47 has been described as cargo protein involved in the trafficking of the mannose-6-phosphate receptor between endosomes and the Golgi complex. Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. PLIN3 has been localized in milk fat globule membranes of human and bovine origin. It has also been described as a placental protein. Increased amounts of TIP47 are secreted into circulation of cervix carcinoma patients. Therefore, TIP47 is probably significant as an oncodevelopmental marker.

Alternate Names

M6PRBP1, Perilipin3, PLIN3, PP17, TIP47

Gene Symbol

PLIN3

Additional Perilipin-3 Products

Product Documents for Human Perilipin-3/TIP47 ELISA Kit (Colorimetric)

Product Datasheets

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COA

Product Specific Notices for Human Perilipin-3/TIP47 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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