PGE2 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA0326

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Saliva, Serum, Tissue culture media, Urine and Whole Blood (100 uL)

Sensitivity

8.26 pg/mL

Assay Range

7.8 to 1000 pg/mL

View all PGE2 ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Description

Quality control test: Standard curve. PGE2 High Sensitivity ELISA Kit is a competitive immunoassay for the quantitative determination of Prostaglandin E2 in biological fluids.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 6.30%

Inter-Assay Precision (Precision between assays) CV% < 10.90%

Recovery for PGE2 ELISA Kit (Colorimetric)

Recovery

Cell Culture Supernatant: 104.40%, Male Serum: 126.10%, Female Serum: 113.70%, Saliva: 123.30%, Urine: 108.90%, Whole Blood: 101.20%

Scientific Data Images for PGE2 ELISA Kit (Colorimetric)

ELISA: PGE2 ELISA Kit (Colorimetric) [KA0326]

ELISA: PGE2 ELISA Kit (Colorimetric) [KA0326]

ELISA: PGE2 ELISA Kit (Colorimetric) [KA0326] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Kit Contents for PGE2 ELISA Kit (Colorimetric)

  • Assay Buffer
  • Goat anti-Mouse IgG Microtiter Plat
  • PGE2 ELISA Conjugate
  • PGE2 HS-ELISA Antibody
  • Plate Sealers
  • pNpp Substrate
  • Prostaglandin E2 Standard
  • Stop Solution
  • Wash Buffer Concentrate

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PGE2

Prostaglandin E2 (PGE2) is an extensively studied prostaglandin owing to its predominance in inflammation, cancer, atherosclerosis, autoimmune disease, and sepsis. Oxidation of arachidonic acid by prostaglandin synthases (COX-1 and COX-2) produces prostaglandin H2 (PGH2), which is further metabolized by PGE synthases into its major product, PGE2. PGE2 mediates autocrine and paracrine signaling by binding to G-protein coupled receptors (EP1, EP2, EP3, EP4) on the cell surface, functioning to modulate phospholipase C and adenylate cyclase activity. PGE2 has been of great interest as a therapeutic target, either by modulation of its synthesis by COX inhibitors (NSAIDS) or by modulation of its receptors by downregulation or binding antagonists. PGE2 production in a variety of tissues has been shown to modulate numerous physiological processes including natriuresis in the kidney, smooth muscle elasticity in the vasculature, and the inflammatory response to damaged tissues by monocytes and macrophages.

Alternate Names

PG E2, PGE 2, PGE2, ProstaglandinE2

Additional PGE2 Products

Product Documents for PGE2 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PGE2 ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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