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Human Phenylalanine Hydroxylase ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81206

Novus Biologicals, part of Bio-Techne
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NBP2-81206

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

46.88 pg/mL

Assay Range

78.13 - 5000 pg/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human Phenylalanine Hydroxylase in samples. No significant cross-reactivity or interference between Human Phenylalanine Hydroxylase and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.22%

Inter-Assay Precision (Precision between assays) CV% < 5.21%

Recovery for Human Phenylalanine Hydroxylase ELISA Kit (Colorimetric)

Recovery

87-108%

Linearity

Human Phenylalanine Hydroxylase ELISA Kit (Colorimetric)

Scientific Data Images for Human Phenylalanine Hydroxylase ELISA Kit (Colorimetric)

Human Phenylalanine Hydroxylase ELISA Kit (Colorimetric)

ELISA: Human Phenylalanine Hydroxylase ELISA Kit (Colorimetric) [NBP2-81206] -

ELISA: Human Phenylalanine Hydroxylase ELISA Kit (Colorimetric) [NBP2-81206] - Standard Curve Reference

Kit Contents for Human Phenylalanine Hydroxylase ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Phenylalanine Hydroxylase

PAH encodes the enzyme phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.

Alternate Names

EC 1.14.16, EC 1.14.16.1, PH, Phe-4-monooxygenase, phenylalanine 4-monooxygenase, phenylalanine hydroxylase, phenylalanine-4-hydroxylase, PKU, PKU1

Gene Symbol

PAH

Additional Phenylalanine Hydroxylase Products

Product Documents for Human Phenylalanine Hydroxylase ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Phenylalanine Hydroxylase ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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