Human PIK3C2A ELISA Kit (Colorimetric)

Name: Human PIK3C2A ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-39340

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-39340

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates and other biological fluids (100 uL)

Sensitivity

1.6 U/L (example only; lot dependent)

Assay Range

3.125 - 200 U/L (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human PIK3C2A ELISA Kit (Colorimetric)

ELISA: Human PIK3C2A ELISA Kit (Colorimetric) [NBP3-39340]

ELISA: Human PIK3C2A ELISA Kit (Colorimetric) [NBP3-39340] - Standard Curve Reference

Kit Contents for Human PIK3C2A ELISA Kit (Colorimetric)

Detection Reagent A
Detection Reagent B
Diluent Buffer
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PIK3C2A

PIK3C2A belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling.

Long Name

Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha

Alternate Names

EC 2.7.1.137, EC 2.7.1.153, EC 2.7.1.154, PI3K-C2-alpha

Gene Symbol

PIK3C2A

Additional PIK3C2A Products

Product Documents for Human PIK3C2A ELISA Kit (Colorimetric)

Product Datasheets

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COA

Product Specific Notices for Human PIK3C2A ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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