Human Podoplanin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81234

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.09 ng/mL

Assay Range

0.16 - 10 ng/mL

View all Podoplanin ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human Podoplanin in samples. No significant cross-reactivity or interference between Human Podoplanin and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.18%

Inter-Assay Precision (Precision between assays) CV% < 5.25%

Recovery for Human Podoplanin ELISA Kit (Colorimetric)

Recovery

92-109%

Linearity

Human Podoplanin ELISA Kit (Colorimetric)

Scientific Data Images for Human Podoplanin ELISA Kit (Colorimetric)

Human Podoplanin ELISA Kit (Colorimetric)

ELISA: Human Podoplanin ELISA Kit (Colorimetric) [NBP2-81234] -

ELISA: Human Podoplanin ELISA Kit (Colorimetric) [NBP2-81234] - Standard Curve Reference

Kit Contents for Human Podoplanin ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Podoplanin

Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is a 38 kDa membrane mucoprotein that was originally detected on the surface of rat glomerular epithelial cells (podocytes) and was found to be linked to flattening of foot processes that occurs in glomerular diseases. Podoplanin shows features of a membrane mucoprotein with several conserved O-glycosylation sites. Currently, it is of unknown biological function. Because heavily O-glycosylated mucoproteins were identified recently as counterreceptors for selectins that mediate adhesion of inflammatory cells, it is possible that podoplanin plays a similar role in lymphatic endothelia.

Long Name

Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A

Alternate Names

Aggrus, Gp38, PDPN, RANDAM-2, T1A-2

Gene Symbol

PDPN

Additional Podoplanin Products

Product Documents for Human Podoplanin ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Podoplanin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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