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Human PON1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81261

Novus Biologicals, part of Bio-Techne
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NBP2-81261

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.09 ng/mL

Assay Range

0.16 - 10 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human PON1 in samples. No significant cross-reactivity or interference between Human PON1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.32%

Inter-Assay Precision (Precision between assays) CV% < 4.95%

Recovery for Human PON1 ELISA Kit (Colorimetric)

Recovery

86-108%

Linearity

Human PON1 ELISA Kit (Colorimetric)

Scientific Data Images for Human PON1 ELISA Kit (Colorimetric)

Human PON1 ELISA Kit (Colorimetric)

ELISA: Human PON1 ELISA Kit (Colorimetric) [NBP2-81261] -

ELISA: Human PON1 ELISA Kit (Colorimetric) [NBP2-81261] - Standard Curve Reference

Kit Contents for Human PON1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PON1

Paraoxonase 1 (PON1) is an arylesterase capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. PON1 primarily hydrolyzes paroxon, an organophosphorus anticholinesterase compound produced in vivo by oxidation of the insecticide parathion, to produce p-nitrophenol. Polymorphisms in this gene are a risk factor in coronary artery disease.

Long Name

Paraoxonase 1

Alternate Names

ESA, K-45

Gene Symbol

PON1

Additional PON1 Products

Product Documents for Human PON1 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human PON1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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