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Human PON2 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81263

Novus Biologicals, part of Bio-Techne
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NBP2-81263

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human PON2 in samples. No significant cross-reactivity or interference between Human PON2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.77%

Inter-Assay Precision (Precision between assays) CV% < 4.66%

Recovery for Human PON2 ELISA Kit (Colorimetric)

Recovery

88-106%

Linearity

Human PON2 ELISA Kit (Colorimetric)

Scientific Data Images for Human PON2 ELISA Kit (Colorimetric)

Human PON2 ELISA Kit (Colorimetric)

ELISA: Human PON2 ELISA Kit (Colorimetric) [NBP2-81263] -

ELISA: Human PON2 ELISA Kit (Colorimetric) [NBP2-81263] - Standard Curve Reference

Kit Contents for Human PON2 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PON2

PON2 encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described.

Long Name

Paraoxonase 2

Alternate Names

A-esterase 2, Serum Aryldialkylphosphatase 2

Gene Symbol

PON2

Additional PON2 Products

Product Documents for Human PON2 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human PON2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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