Human Pyruvate Dehydrogenase E1 beta subunit - Ready-To-Use ELISA Kit (Colorimetric)

Name: Human Pyruvate Dehydrogenase E1 beta subunit - Ready-To-Use ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-40299

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-40299

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates, cell culture supernatants and other biological fluids (100 uL)

Sensitivity

1.2 U/L (example only; lot dependent)

Assay Range

1.56 - 100 U/L (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images

ELISA: Human Pyruvate Dehydrogenase E1 beta subunit - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40299]

ELISA: Human Pyruvate Dehydrogenase E1 beta subunit - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40299] - Standard Curve Reference

Kit Contents

Detection Solution A
Detection Solution B
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Standard Diluent
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Pyruvate Dehydrogenase E1 beta subunit

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3)

Alternate Names

DKFZp564K0164, mitochondrial, pyruvate dehydrogenase (lipoamide) beta, pyruvate dehydrogenase, E1 beta polypeptide

Gene Symbol

PDHB

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Product Documents

Product Datasheets

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COA

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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