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Human RB1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82523

Novus Biologicals, part of Bio-Techne
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NBP2-82523

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.09 ng/mL

Assay Range

0.16 - 10 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human RB1 in samples. No Significant cross-reactivity or interference between Human RB1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.76

Inter-Assay Precision (Precision between assays) CV% < 5.12

Recovery for Human RB1 ELISA Kit (Colorimetric)

Recovery

88-109%

Linearity

Human RB1 ELISA Kit (Colorimetric)

Scientific Data Images for Human RB1 ELISA Kit (Colorimetric)

Human RB1 ELISA Kit (Colorimetric)

ELISA: Human RB1 ELISA Kit (Colorimetric) [NBP2-82523] -

ELISA: Human RB1 ELISA Kit (Colorimetric) [NBP2-82523] - Standard Curve Reference

Kit Contents for Human RB1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: RB1

Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of ts function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

Long Name

Retinoblastoma-associated protein

Alternate Names

OSRC, p105-Rb, pp110

Gene Symbol

RB1

Additional RB1 Products

Product Documents for Human RB1 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human RB1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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