Human RENBP ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA1838

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Plasma, Serum (25 uL)

Sensitivity

0.17 ug/mL

Assay Range

0.195 to 50 ug/mL

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Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Quality control test: Standard curve. RENBP (Human) ELISA Kit is a competitive enzyme immunoassay for the quantitative measurement of human RENBP.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.5%

Inter-Assay Precision (Precision between assays) CV% < 7.2%

Recovery for Human RENBP ELISA Kit (Colorimetric)

Recovery

97%

Scientific Data Images for Human RENBP ELISA Kit (Colorimetric)

ELISA: Human RENBP ELISA Kit (Colorimetric) [KA1838]

ELISA: Human RENBP ELISA Kit (Colorimetric) [KA1838]

ELISA: Human RENBP ELISA Kit (Colorimetric) [KA1838] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Kit Contents for Human RENBP ELISA Kit (Colorimetric)

  • Biotinylated RBP: 1 vial, lyophilized.
  • Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substrate tetramethylbenzidine (8 mL).
  • MIX Diluent Concentrate (10×): A 10-fold concentrated buffered protein base (30 mL).
  • RBP Microplate: A 96-well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody against human RBP.
  • RBP Standard: Human RBP in a buffered protein base (100 µg, lyophilized).
  • Sealing Tapes: Each kit contains 3 pre-cut, pressure-sensitive sealing tapes, which can be cut to fit the format of the individual assay.
  • Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction (12 mL).
  • Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate (80 µL).
  • Wash Buffer Concentrate (20×): A 20-fold concentrated buffered surfactant (30 mL, 1 bottle).

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: RENBP

RENBP, also known as N-acylglucosamine 2-epimerase, is a 49kDa 427 amino acid protein with a shorter 29kDa 254 isoform produced by alternative splicing. RENBP functions as a catalyzer in the conversion of N-acetylglucosamine to N-acetylmannosamine. Current research is being conducted on RENBP in relation to sialuria, Wilms tumor, allergic rhinitis, myopathy, vascular disease, hypertension, nephropathy, proteinuria and aldosteronism. RENBP has been linked to the amino sugar and nucleotide sugar metabolism pathways where it interacts with HEXA, HEXB, GNE, REN and ZBED1.

Alternate Names

AGE, EC 5.1.3.8, GlcNAc 2-epimerase, N-acetyl-D-glucosamine 2-epimerase, N-acylglucosamine 2-epimerase, RBP, renin binding protein, Renin-binding protein, RnBP

Gene Symbol

RENBP

Additional RENBP Products

Product Documents for Human RENBP ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human RENBP ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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