Human Resistin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83744

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), buffered solution (50 uL)

Sensitivity

3.1 pg/mL

Assay Range

31 - 2,000 pg/mL

View all Resistin ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human Resistin positive serum. There was no crossreactivity detected.

Description

This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.1%

Inter-Assay Precision (Precision between assays) CV% < 8.1%

Scientific Data Images for Human Resistin ELISA Kit (Colorimetric)

ELISA: Human Resistin ELISA Kit (Colorimetric) [NBP1-83744]

ELISA: Human Resistin ELISA Kit (Colorimetric) [NBP1-83744]

ELISA: Resistin ELISA Kit [NBP1-83744] - Standard curve

Kit Contents for Human Resistin ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human Resistin
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-human Resistin polyclonal antibody
  • Human Resistin Standard lyophilized, 4000 pg/ml upon reconstitution
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Resistin

Resistin is a hormone secreted by adipose tissue. The length of the resistin pre-peptide in human is 108 aminoacids; the molecular weight is ~12.5 kDa. Resistin was originally found to be produced and released from adipose tissue to serve endocrine functions likely involved in insulin resistance. This idea primarily stems from studies demonstrating that serum resistin levels increase with obesity in several model systems. Since these observations, further research has linked resistin to other physiological systems such as inflammation and energy homeostasis. Current research is proposing to link resistin to inflammation and energy homeostasis, including its alleged role in insulin resistance in obese subjects.

Alternate Names

ADSF, FIZZ3, HXCP1, RETN

Gene Symbol

RETN

Additional Resistin Products

Product Documents for Human Resistin ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Resistin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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