Human RIPK1/RIP1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18223

Novus Biologicals, part of Bio-Techne
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NBP3-18223
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Key Product Details

Sample Type & Volume Required Per Well

Cell lysates, tissue homogenates and other biological fluids (100 ul)

Sensitivity

0.059 ng/ml

Assay Range

0.156-10 ng/ml

View all RIPK1/RIP1 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human RIPK1/RIP1 in the sample, this Human RIPK1/RIP1 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human RIPK1/RIP1 concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10

Inter-Assay Precision (Precision between assays) %CV < 15

Recovery for Human RIPK1/RIP1 ELISA Kit (Colorimetric)

Recovery

84-111%

Scientific Data Images for Human RIPK1/RIP1 ELISA Kit (Colorimetric)

ELISA: Human RIPK1/RIP1 ELISA Kit (Colorimetric) [NBP3-18223]

ELISA: Human RIPK1/RIP1 ELISA Kit (Colorimetric) [NBP3-18223]

ELISA: Human RIPK1/RIP1 ELISA Kit (Colorimetric) [NBP3-18223] - Standard Curve Reference

Kit Contents for Human RIPK1/RIP1 ELISA Kit (Colorimetric)

  • Balance Solution
  • Enzyme Conjugate
  • Microtiter Plate
  • Standards
  • Stop Solution
  • Substrate A
  • Substrate B
  • Wash Solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: RIPK1/RIP1

RIP (Receptor Interacting Protein) is a 74 kD Ser/Thr kinase which interacts with CD95 (Fas/APO1) receptor and the tumor necrosis factor receptor (TNFR1). It is a cell death domain adapter protein which can bind to the adapter proteins TRADD, RAID (CRADD) and TRAF2. RIP contains an N terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C terminal region containing an intracellular death domain motif. RIP activates both p38 MAP Kinase and SAPK families. In vitro, RIP induces apoptosis, as well as SAPK/JNK and NF-kB activation. NF-kB activation through TRADD, TRAF2 and RIP can be triggered also by DR3/APO3 upon activation with APO3/Tweak ligand. DR4 and DR5 also use FADD, TRADD, and RIP in their signal transduction pathways. RIP deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age. RIP possesses kinase activity as it autophosphorylates itself on serine and threonine residues.

Long Name

Receptor (TNFRSF)-Interacting Serine-Threonine Kinase 1

Alternate Names

RIP, RIP1

Gene Symbol

RIPK1

Additional RIPK1/RIP1 Products

Product Documents for Human RIPK1/RIP1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human RIPK1/RIP1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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