SARS-CoV-2 Nucleocapsid ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11405

Novus Biologicals, part of Bio-Techne
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NBP3-11405
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Key Product Details

Sample Type & Volume Required Per Well

Serum, cell culture supernates (100 ul)

Sensitivity

46.68 pg/ml

Assay Range

93.75-6000 pg/ml (example only; lot dependent)

View all SARS-CoV-2 Nucleocapsid ELISA Kits »

Product Specifications

Assay Type

Solid Phase Sandwich ELISA (quantitative)

Kit Type

ELISA Kit (Colorimetric)

Conjugate

HRP

Specificity

Recognizes both recombinant and natural SARS-CoV-2 Coronavirus Nucleocapsid

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.5%

Inter-Assay Precision (Precision between assays) CV% < 5.6%

Recovery for SARS-CoV-2 Nucleocapsid ELISA Kit (Colorimetric)

Recovery

Serum (n=3): 81%
Cell culture supernates (n = 3): 117%

Scientific Data Images for SARS-CoV-2 Nucleocapsid ELISA Kit (Colorimetric)

ELISA: SARS-CoV-2 Nucleocapsid ELISA Kit (Colorimetric) [NBP3-11405]

ELISA: SARS-CoV-2 Nucleocapsid ELISA Kit (Colorimetric) [NBP3-11405]

ELISA: SARS-CoV-2 SARS-CoV-2 Nucleocapsid [NBP3-11405] - Sample standard curve

Kit Contents for SARS-CoV-2 Nucleocapsid ELISA Kit (Colorimetric)

  • 96 well microplate coated with Capture Antibody
  • Color Reagent A
  • Color Reagent B
  • Detection Antibody conjugated to HRP
  • Dilution Buffer Concentrate
  • Sample Lysis Buffer
  • Standard Dilution Buffer
  • Standards
  • Stop Solution
  • Wash Buffer Concentrate

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: SARS-CoV-2 Nucleocapsid

The SARS-CoV-2 Nucleocapsid protein is one of the four major structural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1,2). The nucleocapsid protein is an RNA-binding protein that is essential for viral assembly into a ribonucleoprotein complex and also functions in viral budding (2,3). More specifically, after viral replication and RNA synthesis, the viral genomes that are encapsulated by the nucleocapsid protein will bud into the membrane and help with mature virus formation (2). The nucleocapsid protein is synthesized as 419 amino acids (aa) in length with a theoretical molecular weight of 45.6 kDa (4). Structurally, the nucleocapsid protein contains an RNA-binding domain (N1b), a dimerization domain (N2b), and a few shorter regions (N1a, N2a, and spacer B/N3) (3). It is the N1b domain that is suggested to be involved in RNA binding (3). The nucleocapsid N1b domain contains a beta-hairpin, a loop region, and a beta-sheet core, giving it a right-handed shape (3). Conversely, the N2b domain functions in dimerization which promotes assembly into a helical filament where the dimer interface is made of two beta-strands and an alpha-helix portion (3). Inhibition of nucleocapsid assembly may be a potential target for treating COVID-19 infection.

Following SARS-CoV-2 infection, B cells and T cells display an immune response against nucleocapsid protein and nucleocapsid-specific neutralizing antibodies are produced (5). Interestingly, a study of patients who have recovered from COVID-19 revealed that there were no neutralizing antibodies against nucleocapsid protein present; however, a high presence against neutralizing spike RBD antibodies were detected (5).

References

1. Pillay T. S. (2020). Gene of the month: the 2019-nCoV/SARS-CoV-2 novel coronavirus spike protein. Journal of Clinical Pathology. https://doi.org/10.1136/jclinpath-2020-206658

2. Malik Y. A. (2020). Properties of Coronavirus and SARS-CoV-2. The Malaysian Journal of Pathology.

3. Zhu, G., Zhu, C., Zhu, Y., & Sun, F. (2020). Minireview of progress in the structural study of SARS-CoV-2 proteins. Current Research in Microbial Sciences. https://doi.org/10.1016/j.crmicr.2020.06.003

4. Uniprot (P0DTC9)

5. Shah, V. K., Firmal, P., Alam, A., Ganguly, D., & Chattopadhyay, S. (2020). Overview of Immune Response During SARS-CoV-2 Infection: Lessons From the Past. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2020.01949

Alternate Names

2019-nCoV N Protein, 2019-nCoV Nucleocapsid Protein, COVID-19 N protein, COVID-19 nucleocapsid protein, Human coronavirus Nucleoprotein, nucleocapsid phosphoprotein, Nucleocapsid protein, Nucleoprotein, ORF9; structural protein, SARS-CoV-2, SARS-CoV-2 Nucleocapsid protein, SARS-CoV-2 N protein, SARSCoV2 Nucleoprotein, Severe Acute Respiratory Syndrome Coronavirus 2 Nucleocapsid Protein

Gene Symbol

N

Additional SARS-CoV-2 Nucleocapsid Products

Product Documents for SARS-CoV-2 Nucleocapsid ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SARS-CoV-2 Nucleocapsid ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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