Human Semaphorin 4A ELISA Kit (Colorimetric)

Name: Human Semaphorin 4A ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-40458

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-40458

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.116 ng/mL (example only; lot dependent)

Assay Range

0.312 - 20 ng/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human Semaphorin 4A ELISA Kit (Colorimetric)

ELISA: Human Semaphorin 4A ELISA Kit (Colorimetric) [NBP3-40458]

ELISA: Human Semaphorin 4A ELISA Kit (Colorimetric) [NBP3-40458] - Standard Curve Reference

Kit Contents for Human Semaphorin 4A ELISA Kit (Colorimetric)

Detection Reagent A
Detection Reagent B
Diluent Buffer
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Semaphorin 4A

Semaphorins are a family of cell surface and secreted proteins that are conserved from insects to humans. Members of this family of proteins are approximately 750 amino acids in length (including signal sequences) and are defined by a conserved extracellular "Semaphorin" domain of approximately 500 amino acids containing 14-16 cysteines, blocks of conserved sequences and no obvious repeats. The transmembrane semaphorins are characterized by an additional 80 amino acid transmembrane domain and an 80-110 amino acid cytoplasmic domain. Secreted and cell-bound semaphorins chemically attract and repel the growth of neural axons, guiding the development of intricate networks of neural tissue. Semaphorin 4A (SEMA4A), also designated Semaphorin B, is a type I membrane protein. The SEMA4A gene encoding the protein localizes to chromosome 1q22. SEMA4A provides signals to specify territories inaccessible for growing neurons, inhibiting axonal extension.

Alternate Names

CORD10, SEMA4A, SEMAB, SEMB

Gene Symbol

SEMA4A

Additional Semaphorin 4A Products

Product Documents for Human Semaphorin 4A ELISA Kit (Colorimetric)

Product Datasheets

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COA

Product Specific Notices for Human Semaphorin 4A ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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