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Human SOD3/EC-SOD - Ready-To-Use ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-40143

Novus Biologicals, part of Bio-Techne
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NBP3-40143

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell lysates and other biological fluids (100 uL)

Sensitivity

2.23 ng/mL (example only; lot dependent)

Assay Range

6.25 - 400 ng/mL (example only; lot dependent)

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human SOD3/EC-SOD - Ready-To-Use ELISA Kit (Colorimetric)

Human SOD3/EC-SOD - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human SOD3/EC-SOD - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40143]

ELISA: Human SOD3/EC-SOD - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40143] - Standard Curve Reference

Kit Contents for Human SOD3/EC-SOD - Ready-To-Use ELISA Kit (Colorimetric)

  • Detection Solution A
  • Detection Solution B
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Standard Diluent
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: SOD3/EC-SOD

FUNCTION: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. CATALYTIC ACTIVITY: 2 superoxide + 2 H+ = O2 + H2O2. COFACTOR: Binds 1 copper ion per subunit. COFACTOR: Binds 1 zinc ion per subunit. SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Secreted protein; extracellular space. Note=99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in quilibrium between the plasma and the endothelium. TISSUE SPECIFICITY: Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid. POLYMORPHISM: The variant Gly-231 which is found in about 2.2% of individual displays a 10-fold increased plasma EC-SOD content due to reduced heparin-binding affinity and thus the impairment of its binding ability to endothelial cell surface. SIMILARITY: Belongs to the Cu-Zn superoxide dismutase family.

Long Name

Superoxide Dismutase-3

Alternate Names

EC-SOD, ECSOD

Gene Symbol

SOD3

Additional SOD3/EC-SOD Products

Product Documents for Human SOD3/EC-SOD - Ready-To-Use ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human SOD3/EC-SOD - Ready-To-Use ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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