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Mouse SPARC ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68075

Novus Biologicals, part of Bio-Techne
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NBP2-68075

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

1.88 ng/mL

Assay Range

3.13 - 200 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This kit recognizes Mouse ON in samples. No significant cross-reactivity or interference between Mouse ON and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.79%

Inter-Assay Precision (Precision between assays) CV% < 5.11%

Recovery for Mouse SPARC ELISA Kit (Colorimetric)

Recovery

84-105%

Linearity

Mouse SPARC ELISA Kit (Colorimetric)

Scientific Data Images for Mouse SPARC ELISA Kit (Colorimetric)

Mouse SPARC ELISA Kit (Colorimetric)

ELISA: Mouse SPARC ELISA Kit (Colorimetric) [NBP2-68075] -

ELISA: Mouse SPARC ELISA Kit (Colorimetric) [NBP2-68075] - Standard Curve Reference

Kit Contents for Mouse SPARC ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: SPARC

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.

Long Name

Secreted Protein Acidic and Rich in Cysteine

Alternate Names

BM-40, Osteonectin

Gene Symbol

SPARC

Additional SPARC Products

Product Documents for Mouse SPARC ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse SPARC ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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