Human Sphingosine Kinase 1/SPHK1 ELISA Kit (Colorimetric)

Name: Human Sphingosine Kinase 1/SPHK1 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-40102

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-40102

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.114 ng/mL (example only; lot dependent)

Assay Range

0.312 - 20 ng/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human Sphingosine Kinase 1/SPHK1 ELISA Kit (Colorimetric)

ELISA: Human Sphingosine Kinase 1/SPHK1 ELISA Kit (Colorimetric) [NBP3-40102]

ELISA: Human Sphingosine Kinase 1/SPHK1 ELISA Kit (Colorimetric) [NBP3-40102] - Standard Curve Reference

Kit Contents for Human Sphingosine Kinase 1/SPHK1 ELISA Kit (Colorimetric)

Detection Reagent A
Detection Reagent B
Diluent Buffer
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Sphingosine Kinase 1/SPHK1

Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM]

Alternate Names

SK1, SPK1

Gene Symbol

SPHK1

Additional Sphingosine Kinase 1/SPHK1 Products

Product Documents for Human Sphingosine Kinase 1/SPHK1 ELISA Kit (Colorimetric)

Product Datasheets

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COA

Product Specific Notices for Human Sphingosine Kinase 1/SPHK1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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