Human Sulfatase Modifying Factor 1/SUMF1 ELISA Kit (Colorimetric)

Name: Human Sulfatase Modifying Factor 1/SUMF1 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-40140

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-40140

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates and other biological fluids (100 uL)

Sensitivity

0.064 ng/mL (example only; lot dependent)

Assay Range

0.156 - 10 ng/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human Sulfatase Modifying Factor 1/SUMF1 ELISA Kit (Colorimetric)

ELISA: Human Sulfatase Modifying Factor 1/SUMF1 ELISA Kit (Colorimetric) [NBP3-40140]

ELISA: Human Sulfatase Modifying Factor 1/SUMF1 ELISA Kit (Colorimetric) [NBP3-40140] - Standard Curve Reference

Kit Contents for Human Sulfatase Modifying Factor 1/SUMF1 ELISA Kit (Colorimetric)

Detection Reagent A
Detection Reagent B
Diluent Buffer
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Sulfatase Modifying Factor 1/SUMF1

Sulfatases catalyze the hydrolysis of sulfate esters such as glycosaminoglycans, sulfolipids, and steroid sulfates. C-alpha-formylglycine (FGly), the catalytic residue in the active site of eukaryotic sulfatases, is posttranslationally generated from a cysteine by SUMF1, the FGly-generating enzyme (FGE), in the endoplasmic reticulum (ER). The genetic defect of FGly formation caused by mutations in the SUMF1 gene results in multiple sulfatase deficiency (MSD; MIM 272200), a lysosomal storage disorder (Roeser et al., 2006 [PubMed 16368756]).[supplied by OMIM]

Alternate Names

FGE1

Gene Symbol

SUMF1

Additional Sulfatase Modifying Factor 1/SUMF1 Products

Product Documents for Human Sulfatase Modifying Factor 1/SUMF1 ELISA Kit (Colorimetric)

Product Datasheets

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COA

Product Specific Notices for Human Sulfatase Modifying Factor 1/SUMF1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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